Trichuris suis L3 larvae mRNA library - SRA

SRX386007: Trichuris suis L3 larvae mRNA library
1 ILLUMINA (Illumina HiSeq 2000) run: 20.6M spots, 3.7G bases, 2.2Gb downloads

Design: Total RNAs from larvae at 18 days post-infection (L3: n = 15,000 from 4 pigs) were purified using the TriPure reagent (Roche), and both yield and quality were verified using the 2100 BioAnalyzer. Polyadenylated (polyA ) RNA was purified from 10 μg of total RNA for each library construction using Sera-mag oligo(dT) beads, fragmented to a size of 300-500 bp, reverse-transcribed using random hexamers, end-repaired and adapter-ligated, according to the manufacturer’s protocol (Illumina). Ligated products of ~400 base pairs (bp) were excised from agarose and then PCR-amplified (15 cycles), as recommended. Products were purified over a MinElute column (Qiagen) and subjected to paired-end RNA-seq using HiSeq 2000 (Illumina) and assessed for quality and adapter sequence. Following sequencing, all raw reads were trimmed of Illumina adapters, and filtered for length (≥ 40 nt) and low-quality data (reads containing 4 or more consecutive bases with a PHRED quality of < 20).

Submitted by: UNIVERSITY OF MELBOURNE

Study: Trichuris suis isolate:DCEP-RM93M Genome sequencing and assembly

PRJNA208415 • SRP033425 • All experiments • All runs

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Draft whole genome sequence of a male Trichuris suis individual

Sample: Generic sample from Trichuris suis

SAMN02423290 • SRS510026 • All experiments • All runs

Organism: Trichuris suis

Library:

Name: Trichuris suis L3 larvae mRNA library

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 20.6M spots, 3.7G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR1041652	20,603,515	3.7G	2.2Gb	2015-07-22

ID:: 553859

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Sequence Read Archive

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